Research Objective
The Study indicates that research provides chemo preventive effects
Significant reduction in cancerous cells
Indicative suppression of growth of tumor
Quantifying and analyzing DNA damage in individual cells

Abstract
PHPC Compound is the patent and proprietary medicine developed by Benmoon Pharma Research Pvt Ltd., Ahmedabad. The entire study was planned to do evaluation of efficacy and safety of PHPC Compound in experimental animal’s models. First it was screened in-vitro using breast cancer cell line using MTT assay method, wherein it has shown significant reduction in viable cancerous cells.
After promising result in in-vitro study, safety was checked using Acute toxicity study (OECD 425), wherein it was found safe till the dose of 2000-5000mg/kg, orally.

Further, for long term usage of this formulation, its sub-chronic (90 days) oral toxicity study was done (AYUSH & OECD 408 guideline) in rats and guinea pigs, wherein it shows no significant toxic sign, symptoms and in histology too. To check whether it retards toxic effects against chemotherapeutic agents induced toxicity, it was evaluated against 5-FU model, wherein also it has shown chemo-preventive activity.

To check its effectiveness against breast cancer, it was further screened against DMBA-induced cancer model, wherein also it has shown significant reduction in occurrence of tumor, reduction in tumor size and volume. DMBA induced breast cancer by DNA mutation and increasing free radicals level. The treatment of PHPC Compound reduces free radical levels as evident by significant increase in SOD and Catalase level. Further, it reduces serum level of SGOT and SGPT level, which usually get secreted during tissue damage.
Further to check the effectiveness of the PHPC Compound on DNA damage in individual cells of cancer cell line. In the present study, in 5-FU (5.0μg/ml ) treated cell line, there was reduction in % Head and increase in % Tail, which indicates DNA damage in L929 and MCF-7 cell line. Wherein in case of Test drug (20, 40 and 60 μg /ml) groups, there was significant increase in % Head and decrease in % Tail, which indicates that test drug provides chemo preventive effects. In brief, PHPC Compound has shown promising level of safety and efficacy in experimental animal models.

Introduction
The purpose of the research study on cancer is to develop safe and effective methods to prevent, detect, diagnose, treat, and, ultimately, cure the collections of diseases we call cancer. Ideas for new cancer research studies are often inspired by findings from earlier research, research on other cancers, and even findings from research done on other diseases, such as diabetes or immune disorders. Its aim is to reduce the number of deaths from cancer.
The research reports that covers cytotoxicity study evaluated for cytotoxicity against human adenocarcinoma cell for performing Anti - Cancer Activity effect on cellular DNA damage with his research called PHPC Compound has shown significant reduction in occurrence of tumor, reduction in tumor size and volume, having conducting clinical researches, investigating methods of prevention, diagnosis & treatment studies for several years . The results revealed that the formulation showed significant cytotoxicity on cell line. Overall, the cell growth inhibition by the PHPC observed in this study synergistic response rates. In brief, PHPC Compound has showing promising level of results in experimental models. To check the effect of PHPC Compound on cellular damage against normal cell line and breast cancer cell line using commet assay method. Cell count, Splitting of cells, ratio analysis of the cells and cells viability following the procedure mentioned allow the cell to grow and divided into groups.

To check the effect of formulation PHPC Compound on cellular DNA damage against normal celline and against breast cancer cell line MCF 7 using single cell gel electrophoresis technique commet assay. The scope covers comet assay of given test samples as per in house method. Evaluation of DNA damage for visualization of DNA damange, observations are made of SyBR-stained DNA using a 40x objective on a fluoroscent microscope.
Women breast cancer is the most important cause of mortality in the world. Now a day, some cytotoxic agents are used for its treatment including 5- flurouracil, doxorubicin, daunorubicin, bleomycin, and cisplastin. However, they are costly and known to induce several side effects such as myelosuppression, anemia, and most importantly the generation of cellular resistance. For this, it is important to find alternative therapies or drugs to overcome these drawbacks. Therefore in the present study,the polyherbal formulation screened for its anticancer activity using MCF-7 cell lines. The formulation PHPC Compound was subjected to cell proliferation assay by MTT. The results revealed that the formulation showed significant cytotoxicity on MCF-7 cell line. Overall, the cell growth inhibition by the polyherbal formulation observed in this study synergistic response rates.

Summary of Findings
Mammary tumors were successfully induced by oral administration. PHPC Compound produced significant anti-cancer activity, which was evident by significant reduction in incidence of solid tumor in PHPC Compound treated group. This was clearly indicated by means of results of tumor as compared to diseases control group and histopathology of mammary gland. PHPC Compound significantly increased the level of antioxidant enzymes like SOD and catalase, which were reduced in case of diseased controlled groups. This indicates chemo-protective effect of PHPC Compound.