Research Objective
The Study indicates that research provides chemo preventive effects
Significant reduction in cancerous cells
Indicative suppression of growth of tumor
Quantifying and analyzing DNA damage in individual cells

Abstract
PHPC Compound is the patent and proprietary medicine developed by Benmoon Pharma Research Pvt Ltd., Ahmedabad. The entire study was planned to do evaluation of efficacy and safety of PHPC Compound in experimental animal’s models. First it was screened in-vitro using breast cancer cell line using MTT assay method, wherein it has shown significant reduction in viable cancerous cells.
After promising result in in-vitro study, safety was checked using Acute toxicity study (OECD 425), wherein it was found safe till the dose of 2000-5000mg/kg, orally.

Further, for long term usage of this formulation, its sub-chronic (90 days) oral toxicity study was done (AYUSH & OECD 408 guideline) in rats and guinea pigs, wherein it shows no significant toxic sign, symptoms and in histology too. To check whether it retards toxic effects against chemotherapeutic agents induced toxicity, it was evaluated against 5-FU model, wherein also it has shown chemo-preventive activity.

To check its effectiveness against breast cancer, it was further screened against DMBA-induced cancer model, wherein also it has shown significant reduction in occurrence of tumor, reduction in tumor size and volume. DMBA induced breast cancer by DNA mutation and increasing free radicals level. The treatment of PHPC Compound reduces free radical levels as evident by significant increase in SOD and Catalase level. Further, it reduces serum level of SGOT and SGPT level, which usually get secreted during tissue damage.
Further to check the effectiveness of the PHPC Compound on DNA damage in individual cells of cancer cell line. In the present study, in 5-FU (5.0μg/ml ) treated cell line, there was reduction in % Head and increase in % Tail, which indicates DNA damage in L929 and MCF-7 cell line. Wherein in case of Test drug (20, 40 and 60 μg /ml) groups, there was significant increase in % Head and decrease in % Tail, which indicates that test drug provides chemo preventive effects. In brief, PHPC Compound has shown promising level of safety and efficacy in experimental animal models.

Introduction
The purpose of the research study on cancer is to develop safe and effective methods to prevent, detect, diagnose, treat, and, ultimately, cure the collections of diseases we call cancer. Ideas for new cancer research studies are often inspired by findings from earlier research, research on other cancers, and even findings from research done on other diseases, such as diabetes or immune disorders. Its aim is to reduce the number of deaths from cancer.
The research reports that covers cytotoxicity study evaluated for cytotoxicity against human adenocarcinoma cell for performing Anti - Cancer Activity effect on cellular DNA damage with his research called PHPC Compound has shown significant reduction in occurrence of tumor, reduction in tumor size and volume, having conducting clinical researches, investigating methods of prevention, diagnosis & treatment studies for several years . The results revealed that the formulation showed significant cytotoxicity on cell line. Overall, the cell growth inhibition by the PHPC observed in this study synergistic response rates. In brief, PHPC Compound has showing promising level of results in experimental models. To check the effect of PHPC Compound on cellular damage against normal cell line and breast cancer cell line using commet assay method. Cell count, Splitting of cells, ratio analysis of the cells and cells viability following the procedure mentioned allow the cell to grow and divided into groups.

To check the effect of formulation PHPC Compound on cellular DNA damage against normal celline and against breast cancer cell line MCF 7 using single cell gel electrophoresis technique commet assay. The scope covers comet assay of given test samples as per in house method. Evaluation of DNA damage for visualization of DNA damange, observations are made of SyBR-stained DNA using a 40x objective on a fluoroscent microscope.
Women breast cancer is the most important cause of mortality in the world. Now a day, some cytotoxic agents are used for its treatment including 5- flurouracil, doxorubicin, daunorubicin, bleomycin, and cisplastin. However, they are costly and known to induce several side effects such as myelosuppression, anemia, and most importantly the generation of cellular resistance. For this, it is important to find alternative therapies or drugs to overcome these drawbacks. Therefore in the present study,the polyherbal formulation screened for its anticancer activity using MCF-7 cell lines. The formulation PHPC Compound was subjected to cell proliferation assay by MTT. The results revealed that the formulation showed significant cytotoxicity on MCF-7 cell line. Overall, the cell growth inhibition by the polyherbal formulation observed in this study synergistic response rates.

Summary of Findings
Mammary tumors were successfully induced by oral administration. PHPC Compound produced significant anti-cancer activity, which was evident by significant reduction in incidence of solid tumor in PHPC Compound treated group. This was clearly indicated by means of results of tumor as compared to diseases control group and histopathology of mammary gland. PHPC Compound significantly increased the level of antioxidant enzymes like SOD and catalase, which were reduced in case of diseased controlled groups. This indicates chemo-protective effect of PHPC Compound.

Dr. Sethi at Medanta, The Medicity is the leading Pediatric Nephrologist providing diagnostic and treatment services for children with conditions of the kidney especially Nephrotic syndrome, Glomerular disorders, rare tubular disorders and Chronic Kidney disease. Our team is the leading dialysis and renal transplant service centre for children. Dr. Sethi & team believes in personalized care that focuses on individual patient and family needs. Our team understands the challenges many of our families face when trying to access the care they need. Our caring goes beyond diagnostics and treatment. Our team is nationally recognized for their innovation, experience, dedication and expertise. We have the newest dialysis technology inpatient and outpatient, and provides comprehensive kidney transplant care to children of all ages.

Dr. Sethi leads in care of children with the following disorders:
Pediatric Nephrotic syndrome
Complex recurrent urinary tract disorders
Glomerular disorders
Blood or protein in the urine
Glomerulonephritis
Hemolytic uremic syndrome
Hydronephrosis
Hypertension
Rare tubular disorders
Polycystic kidney diseases
Neurogenic bladder
Renal dysplasia
Renal tubular acidosis
Systemic lupus erythematosus
Vesicoureteral reflux
Acute Kidney Injury
Chronic Kidney disease
Kidney Transplantation- Blood group compatible and incompatible; transplantation in complex renal anomalies

Dr. Sidharth Kumar Sethi
Kidney & Urology Institute

He was trained as a Fellow (International Pediatric Nephrology Association Fellowship) and Senior Resident in Pediatric Nephrology at All India Institute of Medical Sciences and Division of Pediatric Nephrology and Transplant Immunology, Cedars Sinai Medical Centre, Los Angeles, California. He has been actively involved in the care of children with all kinds of complex renal disorders, including nephrotic syndrome, tubular disorders, urinary tract infections, hypertension, chronic kidney disease, and renal transplantation. He has been a part of 8-member writing committee for the guidelines of Steroid Sensitive Nephrotic Syndrome and Expert committee involved in the formulation of guidelines of Pediatric Renal Disorders including Steroid Resistant Nephrotic Syndrome and urinary tract infections. He has more than 30 indexed publications in Pediatric Nephrology and chapters in reputed textbooks including Essential Pediatrics (Editors O.P. Ghai) and “Pediatric Nephrology� (Editors A Bagga, RN Srivastava). He is a part of Editorial Board of “World Journal of Nephrology� and “eAJKD- Web version of American Journal of Kidney Diseases�. He is a reviewer for Pediatric Nephrology related content for various Pediatric and Nephrology journals.

Sebaceous cysts (epidermal cysts) may be a foreign term to you, but many women have indeed experienced them — perhaps without really knowing what they were at the time.
Sebaceous cysts are small lumps or bumps just under the skin. To be more specific, they are closed sacs that contain keratin — a "pasty" or "cheesy" looking protein that often has a foul odor.
Although they are usually found on the face, neck, and trunk, they also occur in the vaginal area or other parts of the genitalia. In cases of the latter, it is not uncommon for women to fear that they are the result of genital herpes. But genital herpes create a blister-type sore that eventually becomes crusty, not just a bump or lump under the skin.

What Causes Sebaceous Cysts?
Sebaceous cysts are often the result of swollen hair follicles or skin trauma.

Symptoms and Signs/Symptoms of Infection

Sebaceous cysts are usually painless, slow-growing, small bumps or lumps that move freely under the skin. It's important not to touch or try to remove the substance inside to prevent tenderness, swelling, and infection of a sebaceous cyst.
Occasionally, infections may occur. Signs or symptoms that may indicate infection of sebaceous cysts include:
Redness
Tenderness
Increased temperature of the skin over the bumps or lumps
Greyish white, cheesy, foul-smelling material draining from the bump or lump

How Are Sebaceous Cysts Diagnosed ?

Sebaceous cysts, to the trained eye, are usually easily diagnosed by their appearance. In some cases, a biopsy may be necessary to rule out other conditions with a similar appearance. You should see your doctor to get a formal diagnosis if you suspect that you have a sebaceous cyst.

Treatments for Sebaceous Cysts

Sebaceous cysts most often disappear on their own and are not dangerous. As stated, however, they may become inflamed and tender. Sometimes sebaceous cysts grow large enough that they may interfere with your everyday life. When this happens, surgical removal may be necessary, and this procedure can be done at your doctor's office. Small inflamed cysts can often be treated with an injection of steroid medications or with antibiotics.

Complications of Sebaceous Cysts

If sebaceous cysts become infected, they can form into painful abscesses.
The surgical removal of a sebaceous cyst that does not involve the excision of the entire sac may cause the cyst to come back (although, recurrence is not usual).
Remember to consult Dr. B C Shah anytime you notice any type of growth, bump, or lump on your body. Although sebaceous cysts are not dangerous, your doctor should examine you to ensure that another more dangerous concern is not present.

- See more at: http://drbcshah.com/sebaceous-cyst-removal/#sthash.FpyyRl4g.dpuf

(Dr. B C Shah recently performed Laparoscopic Cholecystectomy on Mr. N_____ G______ who had 512 stones!)

Mr. N_____ G______ came to me with history of chronic pain in upper abdomen. The pain would get aggravated after meals. His sonography revealed that his gall bladder was distended & full of stones. I performed Laparoscopic Cholecystectomy on him. It was a difficult case as there were lot of adhesions. The gall bladder was delivered successfully It was a pleasant surprise to find 512 stones in the Gall Bladder.

One often wonders as to why patients wait so long. Many times patients come to me with Gall Stones. Often they have only one small stone. The common question asked is "Do I still need surgery for just a small stone?"

As per my observation of last 23 years, one stone or many stones – all have a potential to create complications including even death. Its not just the numbers or size. One small stone can just simply slip into the bile duct and is sufficient to trigger Pancreatitis. I personally know of a patient who developed severe pancreatitis due to a 3 mm small stone. She battled for two months in one of the best hospitals in Mumbai and ultimately died.

In kidney stones, one of the criteria on which the therapy is based is the number of stones and its size. Smaller stones can pass out spontaneously and the patient's problem gets solved naturally. However, this is not the situation with gall stones. A gall stone or its fragment passing out can be dangerous as it can cause blockage of bile in liver or swelling in pancreas. Such complications can occur any time and no doctor on earth can predict when this will occur.

Many patients wait for the stones to grow and multiply. Surely this has a potential of inviting big untimely trouble. Don't wait. There are no warning signs.As far as records go, the largest number of gallstones removed was 3,110 in an open surgery in Britain in 1983, reported in the Guinness Book of World Records.

- See more at: http://drbcshah.com/512-stones-found-in-gall-bladder/