Menstruation Problems In Female
Amenorrhea
The absence of menstrual period in women is called Amenorrhea. It is of two types.

1. Primary Amenorrhea
The failure of menses in women even after turning up to 16 years of age, is called as primary amenorrhea.

Causes
• Chromosomal or genetic abnormalities and mainly Turner syndrome. • Uterus structural defects. • Uterus infection. • Cystic fibrosis. • Cushing’s syndrome. • Imbalance in hormones. • Mainly adrenal gland problems. • Intake of Medicines for Cancer disease treatment and anti-depressive.

2. Secondary Amenorrhea
The stop of regular and normal menstrual cycles after the pregnancy for 3 months or more is called as Secondary amenorrhea.

Causes
• Pregnancy. • Breastfeeding and menopause. • Pituitary gland. • Thyroid problems. • Intake of contraception medicines and few antidepressants medicines. • PCOD (Polycystic Ovary Disease). • Strenuous exercise. • Uterus related operations.

Oligomenorrhea
It occurs in women of childbearing age. Some variation in menstruation is normal. A woman who regularly goes over 35 to 40 days without menstruating may be diagnosed with Oligomenorrhea. Light or infrequent menstrual periods less than 35 to 40 milli is also called as Oligomenorrhea.

Causes
• PCOD. • Formation of pituitary glands tumours. • The cause of Oligomenorrhea is mainly seen during the end of menstruation period.

Metrorrhagia
The occurrence of menses period between two menstruation cycles is called as metrorrhagia. Mainly during the release of egg this metrorrhagia problem is seen.

Causes
• Adenomas. • Endometriosis. • Ectopic pregnancy. • Hormones imbalance. • Intake of contraception medicines. • Usage of contraception equipment (IUD).

Menorrhagia
The abnormally heavy bleeding at menstruation for more than normal 5 days is called as Menorrhagia.

Causes
• Uterine fibroids. • PCOD problems. • Thyroid problems. • Endometriosis. • Cancer in the Uterus.

Dysmenorrhea
The Painful menstruation, typically involving abdominal cramps that effect daily activities of women is called as Dysmenorrhea. It is mainly seen either before the few days of the menstruation periods or during the first three days of menstruation period.

Causes
• Endometriosis. • Tumors in hysterectomy. • Ovarian water cysts. • Hormones imbalance.

GENERAL SYMPTOMS OF THE DISEASE
Menopausal Symptoms Peri-menopausal and menopausal symptoms can appear at any time of life, although usually their onset begins in a woman’s late forties or early fifties. Menopausal symptoms are clearly delineated in most women, and consist of one or more of the following problems - some patients even present with all of the below symptoms. • Anemia, Weakness,* Feeling of intense heat in your body on some occasions • Profuse sweating at night • Excessive falling of hair, • Irregular menstrual periods with excessive or scanty bleeding, • Burning and increased frequency of micturation, • Vaginal dryness and itching • Joint pain, • Edema, • Irritable nature, • Sleeplessness Lassitude.

INDICATION
• Leucorrhoea • Dysmenorrhea • Amenorrhoea • Metrorrhagia • Oligomenorrhea • Menorrhagia • Low Back Pain

Benefits Of Gyneza
• it is helpful in dealing with health of female reproductive system • Increase fertility in females • Releases stress • Enhances immunity which helps to optimize overall health • Helpful in leucorrhoea • Increase Stamina • scanty menstruation • menopause

COMPOSITION
Ashoka, Dashmoola, Lodhra, Guduchi, Punarnava, Shatavari, Musta, Anantmoola, Triphala, Jatamansi, Pippalimoola, Trikatu, Aal, Methika, Ashwagandha

Ashoka
Ashoka bark is considered as the uterine tonic and uterine sedative as it helps in reducing the pain and bleeding during periods.

Dashmoola
These magic herbs and spices soothe the digestive process. It is used in the treatment of anemia, .

Lodhra
• Lodhra is considered useful in managing female disorders such as leucorrhea (excessive vaginal discharge) which is caused by vaginal infections as it has antimicrobial and anti-inflammatory properties. • It is a well known coagulant (coagulation is a process by which the blood forms clots to block and then heal a wound/cut to stop the bleeding). It is therefore used in the treatment of bleeding disorders . Lodhra is especially helpful in inflammation of the uterus.

Punarnava
Thus traditional herbs are used in gynecological disorders Ovulation problems due to polycystic ovarian syndrome.

Shatavari
Shatavari might be beneficial in the management of uterine bleeding or heavy menstrual bleeding. It acts as a primary uterine tonic. It helps to balance and strengthen the menstrual system.

Musta
Musta is great for your digestive and urinary system. Motha (nutgrass) in Ayurveda used to treat various female disorders.

Anantmool
It is used for the management of many gynecological problems in females such as menorrhagia.

Triphala
Triphala is a herbal medicine that has been widely used for treating a variety of ailments. This study aims to systematically analyze the antitumor effects of Triphala on gynecological cancers.

Jatamansi
It is recommended for gynecological problems especially in frequent abortions.

Pippalimoola
It is used to treat semen defects and gynecological problems.

Trikatu
Trikatu gives strength to the reproductive system, used to treat obesity as it keeps a check on food cravings, works as anti-inflammatory and analgesic due to the presence of Sunthi in it which works as Vata Shamaka, that is, it balances the aggravated Vata Dosha which is mainly responsible for all kind of pains.

Trikatu
Trikatu gives strength to the reproductive system, used to treat obesity as it keeps a check on food cravings, works as anti-inflammatory and analgesic due to the presence of Sunthi in it which works as Vata Shamaka, that is, it balances the aggravated Vata Dosha which is mainly responsible for all kind of pains.

Aal (Noni)
Noni (Morinda citrifolia) has been used for many years as an anti-inflammatory agent. & gynecological problem that a?ects adolescents and is reported to have many beneficial properties

Methika
Reduces pain in women with painful menstrual periods.

Ashwagandha
Ashwagandha is considered one ... as a blood tonic, especially in gynecological disorders including anemia.

Dosage
2 teaspoon diluted with 100ml water twice a day for 15 days to 3 months.

Research Objective
The Study indicates that research provides chemo preventive effects
Significant reduction in cancerous cells
Indicative suppression of growth of tumor
Quantifying and analyzing DNA damage in individual cells

Abstract
PHPC Compound is the patent and proprietary medicine developed by Benmoon Pharma Research Pvt Ltd., Ahmedabad. The entire study was planned to do evaluation of efficacy and safety of PHPC Compound in experimental animal’s models. First it was screened in-vitro using breast cancer cell line using MTT assay method, wherein it has shown significant reduction in viable cancerous cells.
After promising result in in-vitro study, safety was checked using Acute toxicity study (OECD 425), wherein it was found safe till the dose of 2000-5000mg/kg, orally.

Further, for long term usage of this formulation, its sub-chronic (90 days) oral toxicity study was done (AYUSH & OECD 408 guideline) in rats and guinea pigs, wherein it shows no significant toxic sign, symptoms and in histology too. To check whether it retards toxic effects against chemotherapeutic agents induced toxicity, it was evaluated against 5-FU model, wherein also it has shown chemo-preventive activity.

To check its effectiveness against breast cancer, it was further screened against DMBA-induced cancer model, wherein also it has shown significant reduction in occurrence of tumor, reduction in tumor size and volume. DMBA induced breast cancer by DNA mutation and increasing free radicals level. The treatment of PHPC Compound reduces free radical levels as evident by significant increase in SOD and Catalase level. Further, it reduces serum level of SGOT and SGPT level, which usually get secreted during tissue damage.
Further to check the effectiveness of the PHPC Compound on DNA damage in individual cells of cancer cell line. In the present study, in 5-FU (5.0μg/ml ) treated cell line, there was reduction in % Head and increase in % Tail, which indicates DNA damage in L929 and MCF-7 cell line. Wherein in case of Test drug (20, 40 and 60 μg /ml) groups, there was significant increase in % Head and decrease in % Tail, which indicates that test drug provides chemo preventive effects. In brief, PHPC Compound has shown promising level of safety and efficacy in experimental animal models.

Introduction
The purpose of the research study on cancer is to develop safe and effective methods to prevent, detect, diagnose, treat, and, ultimately, cure the collections of diseases we call cancer. Ideas for new cancer research studies are often inspired by findings from earlier research, research on other cancers, and even findings from research done on other diseases, such as diabetes or immune disorders. Its aim is to reduce the number of deaths from cancer.
The research reports that covers cytotoxicity study evaluated for cytotoxicity against human adenocarcinoma cell for performing Anti - Cancer Activity effect on cellular DNA damage with his research called PHPC Compound has shown significant reduction in occurrence of tumor, reduction in tumor size and volume, having conducting clinical researches, investigating methods of prevention, diagnosis & treatment studies for several years . The results revealed that the formulation showed significant cytotoxicity on cell line. Overall, the cell growth inhibition by the PHPC observed in this study synergistic response rates. In brief, PHPC Compound has showing promising level of results in experimental models. To check the effect of PHPC Compound on cellular damage against normal cell line and breast cancer cell line using commet assay method. Cell count, Splitting of cells, ratio analysis of the cells and cells viability following the procedure mentioned allow the cell to grow and divided into groups.

To check the effect of formulation PHPC Compound on cellular DNA damage against normal celline and against breast cancer cell line MCF 7 using single cell gel electrophoresis technique commet assay. The scope covers comet assay of given test samples as per in house method. Evaluation of DNA damage for visualization of DNA damange, observations are made of SyBR-stained DNA using a 40x objective on a fluoroscent microscope.
Women breast cancer is the most important cause of mortality in the world. Now a day, some cytotoxic agents are used for its treatment including 5- flurouracil, doxorubicin, daunorubicin, bleomycin, and cisplastin. However, they are costly and known to induce several side effects such as myelosuppression, anemia, and most importantly the generation of cellular resistance. For this, it is important to find alternative therapies or drugs to overcome these drawbacks. Therefore in the present study,the polyherbal formulation screened for its anticancer activity using MCF-7 cell lines. The formulation PHPC Compound was subjected to cell proliferation assay by MTT. The results revealed that the formulation showed significant cytotoxicity on MCF-7 cell line. Overall, the cell growth inhibition by the polyherbal formulation observed in this study synergistic response rates.

Summary of Findings
Mammary tumors were successfully induced by oral administration. PHPC Compound produced significant anti-cancer activity, which was evident by significant reduction in incidence of solid tumor in PHPC Compound treated group. This was clearly indicated by means of results of tumor as compared to diseases control group and histopathology of mammary gland. PHPC Compound significantly increased the level of antioxidant enzymes like SOD and catalase, which were reduced in case of diseased controlled groups. This indicates chemo-protective effect of PHPC Compound.